通过对帕氏衣属和鳞衣属异多糖的结构表征研究地衣化真菌的化学型意义。
Chemotypes significance of lichenized fungi by structural characterization of heteropolysaccharides from the genera Parmotrema and Rimelia.
作者信息
Carbonero Elaine Rosechrer, Mellinger Caroline Grassi, Eliasaro Sionara, Gorin Philip Albert James, Iacomini Marcello
机构信息
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, C.P. 19046, CEP 81531-990 Curitiba, PR, Brazil.
出版信息
FEMS Microbiol Lett. 2005 May 15;246(2):273-8. doi: 10.1016/j.femsle.2005.04.019.
Galactoglucomannans were isolated from the lichenized fungi of the genus Parmotrema (Parmotrema austrosinense, Parmotrema delicatulum, Parmotrema mantiqueirense, Parmotrema schindlerii, and Parmotrema tinctorum and that of Rimelia (Rimelia cetrata and Rimelia reticulata) via successive hot alkaline extraction and precipitation with Fehling solution. The structure of each polysaccharide was investigated using 13C NMR and HSQC-DEPT spectroscopy, methylation analysis, and HPSEC-MALLS. The galactoglucomannans had a (1-->6)-linked main chain of alpha-Manp units, substituted preferentially at O-2 and O-4 by alpha-Galp and beta-Galp nonreducing end-units, respectively. The C-1 region of the 13C NMR spectra of these heteropolysaccharides is typical of the lichen species, and is an additional tool in lichenized fungi classification.
通过连续的热碱性提取和用费林溶液沉淀，从盾状衣属（南方盾状衣、细裂盾状衣、曼蒂基拉盾状衣、辛氏盾状衣和染色盾状衣）和网衣属（鲸背网衣和网纹网衣）的地衣化真菌中分离出半乳葡甘露聚糖。使用13C NMR和HSQC-DEPT光谱、甲基化分析和HPSEC-MALLS研究了每种多糖的结构。半乳葡甘露聚糖具有由α-Manp单元组成的(1→6)连接的主链，分别在O-2和O-4位置优先被α-Galp和β-Galp非还原末端单元取代。这些杂多糖的13C NMR光谱的C-1区域是地衣物种的典型特征，是地衣化真菌分类的一个额外工具。
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