对乙酰氨基酚与对氨基酚在毛细管区带电泳中的迁移行为及分离：基于对乙酰氨基酚的药物分析
Migration behaviour and separation of acetaminophen and p-aminophenol in capillary zone electrophoresis: analysis of drugs based on acetaminophen.
作者信息
Pérez-Ruiz Tomás, Martínez-Lozano Carmen, Tomás Virginia, Galera Raquel
机构信息
Department of Analytical Chemistry, University of Murcia, Campus de Espinardo, 30071 Murcia, Spain.
出版信息
J Pharm Biomed Anal. 2005 Jun 1;38(1):87-93. doi: 10.1016/j.jpba.2004.12.014. Epub 2005 Jan 21.
The migration behaviour of acetaminophen and p-aminophenol was investigated by capillary electrophoresis. The influence of different parameters (pH, nature and concentration of the running buffer and applied voltage) on the migration time, peak symmetry, efficiency and resolution was systematically investigated. The two analytes can be well separated within 4 min in a 57 cm fused-silica capillary at a separation voltage of 18 kV in a 50mM borate buffer adjusted to pH 9.5. Correlation coefficients for calibration curves in the range 0.2-200 microg ml-1 for acetaminophen and 0.3-3 microg ml-1 for p-aminophenol were higher than 0.999. The sensitivity of detection is 4.2 ng ml-1 for acetaminophen and 11.2 ng ml-1 for p-aminophenol. The method was applied to the analysis of various commercially available acetaminophen dosage forms with recoveries of 98.4-100.7%.
采用毛细管电泳法研究了对乙酰氨基酚和对氨基酚的迁移行为。系统研究了不同参数（pH值、运行缓冲液的性质和浓度以及施加电压）对迁移时间、峰对称性、效率和分辨率的影响。在50mM硼酸盐缓冲液（pH值调至9.5）中，于18 kV的分离电压下，在57 cm的熔融石英毛细管中，两种分析物可在4分钟内得到良好分离。对乙酰氨基酚在0.2 - 200 μg ml-1范围内以及对氨基酚在0.3 - 3 μg ml-1范围内的校准曲线相关系数均高于0.999。对乙酰氨基酚的检测灵敏度为4.2 ng ml-1，对氨基酚的检测灵敏度为11.2 ng ml-1。该方法应用于多种市售对乙酰氨基酚剂型的分析，回收率为98.4 - 100.7%。
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