Schirenbeck Antje, Bretschneider Till, Arasada Rajesh, Schleicher Michael, Faix Jan
A. Butenandt-Institut/Zellbiologie, Ludwig-Maximilians-Universität, Schillerstr. 42, 80336 München, Germany.
Nat Cell Biol. 2005 Jun;7(6):619-25. doi: 10.1038/ncb1266. Epub 2005 May 22.
Formins have important roles in the nucleation of actin and the formation of linear actin filaments, but their role in filopodium formation has remained elusive. Dictyostelium discoideum Diaphanous-related formin dDia2 is enriched at the tips of filopodia and interacts with profilin II and Rac1. An FH1FH2 fragment of dDia2 nucleated actin polymerization and removed capping protein from capped filament ends. Genetic studies showed that dDia2 is important for cell migration as well as the formation, elongation and maintenance of filopodia. Here we provide evidence that dDia2 specifically controls filopodial dynamics by regulating actin turnover at the barbed ends of actin filaments.
formin蛋白在肌动蛋白的成核及线性肌动蛋白丝的形成过程中发挥重要作用，但其在丝状伪足形成中的作用仍不清楚。盘基网柄菌中与Diaphanous相关的formin蛋白dDia2在丝状伪足尖端富集，并与profilin II和Rac1相互作用。dDia2的一个FH1FH2片段引发肌动蛋白聚合，并从加帽的丝端去除加帽蛋白。遗传学研究表明，dDia2对于细胞迁移以及丝状伪足的形成、伸长和维持都很重要。在此，我们提供证据表明，dDia2通过调节肌动蛋白丝末端的肌动蛋白周转来特异性地控制丝状伪足的动态变化。
