磁共振成像期间小动物的生理监测。

Physiological monitoring of small animals during magnetic resonance imaging.

作者信息

Mirsattari Seyed M, Bihari Frank, Leung L Stan, Menon Ravi S, Wang Zheng, Ives John R, Bartha Robert

机构信息

Department of Clinical Neurological Sciences, University of Western Ontario, 10-OP3, 339 Windermere Rd, London, Ont., Canada, N6A 5A5.

出版信息

J Neurosci Methods. 2005 Jun 15;144(2):207-13. doi: 10.1016/j.jneumeth.2004.11.019. Epub 2005 Jan 8.

DOI:10.1016/j.jneumeth.2004.11.019

PMID:15910980

Abstract

Maintaining a stable physiologic state is essential when studying animal models of epilepsy with simultaneous electroencephalograph (EEG) and functional magnetic resonance imaging (fMRI) or EEG and magnetic resonance spectroscopy (MRS). To achieve and maintain such stability in rats in the MRI environment, a minimally invasive but comprehensive system was developed to monitor body temperature, heart rate, blood pressure, blood oxygen saturation and end-tidal CO2 (ETCO2) of expired gas. All physiologic parameters were successfully monitored in Sprague-Dawley rats without interfering with EEG recordings during simultaneous fMRI and MRS studies. Body temperature, heart rate, blood pressure, blood oxygen saturation, and ETCO2, were maintained between 36.5 and 37.5 degrees C, 250-450 beats/min, 136+/-17 mmHg, >90%, and 20-35 mmHg, respectively for 6-8 h under inhalational anesthesia. This set-up could be extended to study in vivo applications in other laboratory animals with only minor modifications.

摘要

在利用同步脑电图（EEG）和功能磁共振成像（fMRI）或EEG和磁共振波谱（MRS）研究癫痫动物模型时，维持稳定的生理状态至关重要。为了在MRI环境中使大鼠达到并维持这种稳定性，开发了一种微创但全面的系统来监测体温、心率、血压、血氧饱和度和呼出气体的呼气末二氧化碳（ETCO2）。在同步进行fMRI和MRS研究期间，所有生理参数均在Sprague-Dawley大鼠中成功监测到，且未干扰EEG记录。在吸入麻醉下，体温、心率、血压、血氧饱和度和ETCO2分别维持在36.5至37.5摄氏度、250 - 450次/分钟、136±17 mmHg、>90%和20 - 35 mmHg之间达6 - 8小时。这种设置只需稍作修改就可扩展到其他实验动物的体内应用研究。