Cavas María, Beltrán David, Navarro José F
Departamento de Psicobiología y Metodología de las Ciencias del Comportamiento, Facultad de Psicología, Universidad de Málaga, 29071 Málaga, Spain.
Toxicol Lett. 2005 Jul 4;157(3):221-32. doi: 10.1016/j.toxlet.2005.02.003. Epub 2005 Apr 15.
Dimethyl sulfoxide (DMSO) is an efficient solvent for water-insoluble compounds, widely used in biological studies and as a vehicle for drug therapy, but few data on its neurotoxic or behavioural effects is available. The aim of this work is to explore DMSO's effects upon sleep/wake states. Twenty male rats were sterotaxically prepared for polysomnography. Four concentrations of DMSO (5%, 10%, 15%, and 20%, in saline) were examined. DMSO or saline were administered intraperitoneally at the beginning of the light period. Three hours of polygraphic recording were evaluated for stages of vigilance after treatment. Sleep/wake parameters and EEG power spectral analyses during sleep were investigated. Results show no significant effect after 5% or 10% DMSO treatment. DMSO 15% increased mean episode duration of light slow wave sleep (SWS), decreasing mean episode duration of deep SWS and of quiet wake (QW). DMSO 20% increased light SWS enhancing number of episodes, while decreased deep SWS mean episode duration. EEG power spectra of sigma and delta activity were also affected by DMSO. Therefore, DMSO at 15% and 20% affects sleep architecture in rats, increasing light SWS and reducing deep SWS. Being aware of DMSO behavioural effects seems important since experimental artefacts caused by DMSO can lead to the erroneous interpretation of results.
二甲基亚砜（DMSO）是一种用于溶解水不溶性化合物的有效溶剂，广泛应用于生物学研究以及作为药物治疗的载体，但关于其神经毒性或行为影响的数据却很少。这项工作的目的是探究二甲基亚砜对睡眠/觉醒状态的影响。对20只雄性大鼠进行立体定位以进行多导睡眠图监测。研究了四种浓度的二甲基亚砜（在生理盐水中分别为5%、10%、15%和20%）。在光照期开始时腹腔注射二甲基亚砜或生理盐水。在治疗后对三小时的多导记录进行警觉阶段评估。研究了睡眠期间的睡眠/觉醒参数和脑电图功率谱分析。结果显示，5%或10%的二甲基亚砜处理后没有显著影响。15%的二甲基亚砜增加了浅慢波睡眠（SWS）的平均发作持续时间，同时减少了深慢波睡眠和安静觉醒（QW）的平均发作持续时间。20%的二甲基亚砜增加了浅慢波睡眠，增加了发作次数，同时减少了深慢波睡眠的平均发作持续时间。西格玛和德尔塔活动的脑电图功率谱也受到二甲基亚砜的影响。因此，15%和20%的二甲基亚砜会影响大鼠的睡眠结构，增加浅慢波睡眠并减少深慢波睡眠。了解二甲基亚砜的行为影响似乎很重要，因为由二甲基亚砜引起的实验假象可能导致对结果的错误解读。
