里氏木霉在玉米纤维组分上生长时的产酶情况
Profile of enzyme production by Trichoderma reesei grown on corn fiber fractions.
作者信息
Li Xin-Liang, Dien Bruce S, Cotta Michael A, Wu Y Victor, Saha Badal C
机构信息
Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, USDA/ARS, 1815 N. University Street, Peoria, Illinois 61604.
出版信息
Appl Biochem Biotechnol. 2005 Spring;121-124:321-34.
Corn fiber is the fibrous by-product of wet-mill corn processing. It typically consists of about 20% starch, 14% cellulose, and 30% hemicellulose in the form of arabinoxylan. Crude corn fiber (CCF) was fractionated into de-starched corn fiber (DSCF), corn fiber with cellulose (CFC) enriched, and corn fiber arabinoxylan (CFAX), and these fractions were evaluated as substrates for enzyme production by Trichoderma reesei. T. reesei QM9414 and Rut C-30 grew on CCF, DSCF, CFC, or CFAX and secreted a number of hydrolytic enzymes. The enzymes displayed synergism with commercial cellulases for corn fiber hydrolysis.
玉米纤维是湿法加工玉米过程中的纤维状副产品。它通常由约20%的淀粉、14%的纤维素和30%呈阿拉伯木聚糖形式的半纤维素组成。粗玉米纤维（CCF）被分离成脱淀粉玉米纤维（DSCF）、富含纤维素的玉米纤维（CFC）和玉米纤维阿拉伯木聚糖（CFAX），并将这些组分作为里氏木霉产酶的底物进行评估。里氏木霉QM9414和Rut C-30在CCF、DSCF、CFC或CFAX上生长，并分泌多种水解酶。这些酶与商业纤维素酶在水解玉米纤维方面表现出协同作用。
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