确认杀鲑气单胞菌在欧洲海鲈（Dicentrarchus labrax）中为病原体，并与鲑科菌株进行系统发育比较。

Confirmation of Piscirickettsia salmonis as a pathogen in European sea bass Dicentrarchus labrax and phylogenetic comparison with salmonid strains.

作者信息

McCarthy U, Steiropoulos N A, Thompson K D, Adams A, Ellis A E, Ferguson H W

机构信息

Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, UK.

出版信息

Dis Aquat Organ. 2005 Apr 18;64(2):107-19. doi: 10.3354/dao064107.

DOI:10.3354/dao064107

PMID:15918473

Abstract

European sea bass Dicentrarchus labrax from the Mediterranean were diagnosed with a severe encephalitis. Rickettsia-like organisms (RLOs) were associated with brain lesions in routine paraffin sections. These were found to share common antigens with the Piscirickettsia salmonis type-strain, LF-89, by indirect fluorescent antibody test (IFAT) and by immunohistochemistry (IHC). In addition, we compared the DNA sequences of the 16S rDNA and 16S-23S internal transcribed spacer region (ITS) with those published for P. salmonis strains and found that the sea bass piscirickettsia-like organism (SBPLO) was another strain of P. salmonis, closely related to the salmonid pathogens. Furthermore, we showed that the SBPLO possessed at least 2 ITS regions, 1 of which contained tRNA genes.

摘要

来自地中海的欧洲海鲈（Dicentrarchus labrax）被诊断患有严重脑炎。在常规石蜡切片中，类立克次氏体生物（RLOs）与脑部病变有关。通过间接荧光抗体试验（IFAT）和免疫组织化学（IHC）发现，这些生物与鲑鱼立克次氏体（Piscirickettsia salmonis）模式菌株LF-89具有共同抗原。此外，我们将16S rDNA和16S-23S内转录间隔区（ITS）的DNA序列与已发表的鲑鱼立克次氏体菌株的序列进行了比较，发现海鲈类立克次氏体生物（SBPLO）是鲑鱼立克次氏体的另一个菌株，与鲑科病原体密切相关。此外，我们还表明，SBPLO至少拥有2个ITS区域，其中1个包含tRNA基因。