Koterov A N, Novoradovskaya N A, Filippovich I V
Institute of Biophysics, USSR Ministry of Public Health, Moscow.
Indian J Biochem Biophys. 1992 Feb;29(1):9-12.
The possible involvement of SSB-proteins in DNA replication in Ehrlich ascites tumour (EAT) has been investigated. A direct relation (the computer-generated correlation coefficient was 0.9) between the SSB-proteins content in chromatin and intensity of the replicative synthesis of DNA in various preparation of EAT in vivo and in vitro is observed. Addition of exogenous SSB-proteins to the permeable EAT cells has been found to increase the replicative synthesis. Although eukaryotic SSB-proteins are not complete analogs of the prokaryotic SSB-proteins, they evidently participate in DNA replication in eukaryotic cells and possibly are intracellular regulators of proliferation.
已对单链结合蛋白(SSB蛋白)参与艾氏腹水瘤(EAT)DNA复制的可能性进行了研究。观察到在体内和体外的各种EAT制剂中,染色质中SSB蛋白含量与DNA复制合成强度之间存在直接关系(计算机生成的相关系数为0.9)。已发现向可渗透的EAT细胞中添加外源性SSB蛋白可增加复制合成。尽管真核生物的SSB蛋白并非原核生物SSB蛋白的完全类似物,但它们显然参与真核细胞中的DNA复制,并且可能是细胞内增殖的调节因子。