Vetrova E V, Kudryasheva N S, Visser A J W G, van Hoek A
Institute of Biophysics SB RAS, Krasnoyarsk 660036, Russia.
Luminescence. 2005 May-Jun;20(3):205-9. doi: 10.1002/bio.815.
The bioluminescent bacterial enzyme system NAD(P)H:FMN-oxidoreductase-luciferase has been used as a test system for ecological monitoring. One of the modes to quench bioluminescence is the interaction of xenobiotics with the enzymes, which inhibit their activity. The use of endogenous flavin fluorescence for investigation of the interactions of non-fluorescent compounds with the bacterial luciferase from Photobacterium leiognathi and NAD(P)H:FMN-oxidoreductase from Vibrio fischeri has been proposed. Fluorescence spectroscopy methods have been used to study characteristics of endogenous flavin fluorescence (fluorophore lifetime, the rotational correlation time). The fluorescence anisotropy behaviour of FMN has been analysed and compared to that of the enzyme-bound flavin. The fluorescence characteristics of endogenous flavin of luciferase and NAD(P)H:FMN-oxidoreductase have been shown to be applicable in studying enzymes' interactions with non-fluorescent compounds.
生物发光细菌酶系统NAD(P)H:FMN氧化还原酶-荧光素酶已被用作生态监测的测试系统。淬灭生物发光的一种方式是异生物质与酶相互作用，从而抑制其活性。有人提出利用内源性黄素荧光来研究非荧光化合物与来自利氏发光杆菌的细菌荧光素酶以及来自费氏弧菌的NAD(P)H:FMN氧化还原酶之间的相互作用。荧光光谱法已被用于研究内源性黄素荧光的特性（荧光团寿命、旋转相关时间）。分析了FMN的荧光 anisotropy 行为，并与酶结合黄素的荧光 anisotropy 行为进行了比较。已证明荧光素酶和NAD(P)H:FMN氧化还原酶的内源性黄素的荧光特性可用于研究酶与非荧光化合物的相互作用。 （注：“anisotropy”常见释义为“各向异性”，这里结合语境可理解为“荧光各向异性”，但不确定是否准确，你可根据专业知识进一步确认。）
