冲绳连鳍䲗这一具有连续性别转换特征的虾虎鱼中Ad4BP/SF-1的分子克隆与表达

Molecular cloning and expression of Ad4BP/SF-1 in the serial sex changing gobiid fish, Trimma okinawae.

作者信息

Kobayashi Yasuhisa, Sunobe Tomoki, Kobayashi Tohru, Nakamura Masaru, Suzuki Norio, Nagahama Yoshitaka

机构信息

Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus, 3422 Sesoko, Motobu-cho, Okinawa 905-0227, Japan.

出版信息

Biochem Biophys Res Commun. 2005 Jul 15;332(4):1073-80. doi: 10.1016/j.bbrc.2005.05.062.

DOI:10.1016/j.bbrc.2005.05.062

PMID:15925324

Abstract

Involvement of Ad4BP/SF-1 was investigated using the serial sex changing goby Trimma okinawae. First, a cDNA encoding Ad4BP/SF-1 was cloned from ovarian follicles. The open reading frame of goby Ad4BP/SF-1 encodes a protein of 489 amino acids. Ad4BP/SF-1 was expressed in gonadal tissues, brain, and kidney. Second, transcript levels of Ad4BP/SF-1 were measured in the ovary and testis of the same individuals. Expressions were related to sexual phases. Moreover, ovarian expression of Ad4BP/SF-1 increased during vitellogenesis and declined sharply during the post-vitellogenic period. Finally, the expression profile of Ad4BP/SF-1 was measured during serial sex change in the ovary. Ad4BP/SF-1 increased in parallel with the onset of the female-phase and decreased as female became male. Therefore, Ad4BP/SF-1 probably acts as an important indirect regulator of oocyte growth and maturation at female-phase of serial sex changing gobiid fish T. okinawae.

摘要

利用序列性逆转的冲绳小弹涂鱼研究了Ad4BP/SF-1的作用。首先，从卵巢滤泡中克隆出编码Ad4BP/SF-1的cDNA。冲绳小弹涂鱼Ad4BP/SF-1的开放阅读框编码一个489个氨基酸的蛋白质。Ad4BP/SF-1在性腺组织、脑和肾中表达。其次，测定了同一批个体卵巢和精巢中Ad4BP/SF-1的转录水平。其表达与性相有关。此外，Ad4BP/SF-1在卵黄发生期间卵巢表达增加，在卵黄发生后期急剧下降。最后，测定了卵巢序列性逆转过程中Ad4BP/SF-1的表达谱。Ad4BP/SF-1随着雌相的开始而平行增加，随着雌鱼变为雄鱼而减少。因此，Ad4BP/SF-1可能作为序列性逆转的虾虎鱼冲绳小弹涂鱼雌相期卵母细胞生长和成熟的重要间接调节因子。