Roy R, Zaragoza P, Gautier M, Eggen A, Rodellar C
Laboratorio de Genética Bioquímica y Grupos Sanguíneos, Universidad de Zaragoza, Zaragoza, Spain.
Anim Biotechnol. 2005;16(1):1-9. doi: 10.1081/abio-200044295.
Fatness traits, such as fat deposition, carcass composition, fat content, and the percentage of fat in milk, are economically relevant to cattle production. Fatty acid synthase (FASN) and glycerol-3-phosphate acyltransferase mitochondrial (GPAM) are two enzymes that play a central role in de novo lipogenesis. Both could be putative candidate genes for quantitative trait loci (QTL). Several clones containing the fatty acid synthase (FASN) and glycerol-3-phosphate acyltransferase mitochondrial (GPAM) genes were isolated after screening the INRA bovine bacterial artificial chromosome (BAC) library using PCR. Five microsatellite loci were derived from the BAC clones containing the genes of interest with heterozygosity values ranging from 27 to 78%, using DNA samples from the International Bovine Reference Panel (IBRP). The newly developed markers were genotyped on the IBRP animals and on a radiation hybrid panel to compare the obtained linkage and RH maps. Radiation hybrid maps were developed for chromosome BTA19 and BTA26 regions containing FASN and GPAM genes, respectively. The two genes and their associated microsatellite markers were located on the genetic or RH maps or on both. These microsatellite markers could be useful to study the QTL effect on fat synthesis in reference population.
脂肪性状，如脂肪沉积、胴体组成、脂肪含量和乳脂肪百分比，在肉牛生产中具有重要经济意义。脂肪酸合酶（FASN）和线粒体甘油-3-磷酸酰基转移酶（GPAM）是在从头脂肪生成过程中起核心作用的两种酶。二者都可能是数量性状位点（QTL）的候选基因。利用聚合酶链反应（PCR）筛选法国国家农业研究院（INRA）牛细菌人工染色体（BAC）文库后，分离出了几个包含脂肪酸合酶（FASN）和线粒体甘油-3-磷酸酰基转移酶（GPAM）基因的克隆。利用国际牛参考群体（IBRP）的DNA样本，从包含目标基因的BAC克隆中获得了5个微卫星位点，杂合度值在27%至78%之间。在IBRP动物和辐射杂种细胞系上对新开发的标记进行基因分型，以比较获得的连锁图谱和辐射杂种图谱。分别针对包含FASN和GPAM基因的BTA19和BTA26染色体区域构建了辐射杂种图谱。这两个基因及其相关的微卫星标记位于遗传图谱或辐射杂种图谱上，或同时位于二者上。这些微卫星标记对于研究参考群体中QTL对脂肪合成的影响可能有用。
