Panani Anna D, Roussos Charis
Critical Care Department, Research Unit, Medical School of Athens University, Evangelismos Hospital, Ipsilandou 45-47, Athens 10676, Greece.
Cancer Lett. 2006 Apr 8;235(1):130-5. doi: 10.1016/j.canlet.2005.04.010. Epub 2005 May 31.
Ovarian cancer represents the leading cause of death among patients with gynecological cancer. The genetic changes underlying the initiation and progression of ovarian cancer have not been well defined. However, non-random structural chromosomal changes have been identified with common chromosomal breakpoints. We have studied cytogenetically 15 cases of ovarian adenocarcinomas by a direct culture of cancer cells and a G-banding technique investigating the presence of recurrent structural aberrations with common chromosomal breakpoints. Among very complex structural rearrangements found, we could recognize recurrent structural aberrations involving according to frequency chromosomal regions 3p13-14, 11p15, 19q13, 3q21, 11q23, 11q10, 1p13, 1p36, and 17q24-25. Isochromosomes i(5p), i(17q), i(8q) and i(11q) were also observed. Isochromosome i(5p), rarely reported in ovarian cancer was found in seven cases suggesting that it may be a novel recurrent abnormality. Translocations t(1;11), t(3;19), t(3;17), t(7;11) and t(11;17) were also identified. Conventional cytogenetics continues to be valuable detecting the presence of non-random chromosomal breakpoints and facilitating the identification of genes implicated in tumorigenesis.
卵巢癌是妇科癌症患者死亡的主要原因。卵巢癌发生和发展的潜在基因变化尚未明确。然而，已发现具有常见染色体断点的非随机结构染色体变化。我们通过癌细胞直接培养和G显带技术对15例卵巢腺癌进行了细胞遗传学研究，调查具有常见染色体断点的复发性结构畸变的存在情况。在发现的非常复杂的结构重排中，我们能够识别出根据频率涉及染色体区域3p13 - 14、11p15、19q13、3q21、11q23、11q10、1p13、1p36和17q24 - 25的复发性结构畸变。还观察到等臂染色体i(5p)、i(17q)、i(8q)和i(11q)。在7例病例中发现了在卵巢癌中很少报道的等臂染色体i(5p)，这表明它可能是一种新的复发性异常。还鉴定出易位t(1;11)、t(3;19)、t(3;17)、t(7;11)和t(11;17)。传统细胞遗传学在检测非随机染色体断点的存在以及促进鉴定与肿瘤发生相关的基因方面仍然具有重要价值。
