原发性乳腺癌中内皮型一氧化氮合酶表达的免疫组织化学评估

Immunohistochemical evaluation of endothelial nitric oxide synthase expression in primary breast cancer.

作者信息

Loibl S, Strank C, von Minckwitz G, Sinn H-P, Buck A, Solbach C, Strebhardt K, Kaufmann M

机构信息

Department of Gynaecology and Obstetrics, Johann-Wolfgang Goethe-University, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany.

出版信息

Breast. 2005 Jun;14(3):230-5. doi: 10.1016/j.breast.2004.11.005.

DOI:10.1016/j.breast.2004.11.005

PMID:15927832

Abstract

To evaluate the clinical potential of endothelial nitric oxide synthase (e-NOS) in human breast cancer, we performed immunohistochemical (IHC) staining of paraffin-embedded primary breast cancer tissue of 163 patients for e-NOS using a monoclonal antibody. A correlation was found between e-NOS expression and both the classic prognostic factors and survival rates. Under half the patients were premenopausal (38.5%), 61.5% being postmenopausal. The median tumour size was 2 cm; in 41.7% of the patients there was involvement of the axillary lymph nodes. Most (84.1%) of the tumours were hormone receptor positive. e-NOS staining was positive in 62%, most of the positive tumours having weak (32.5%) or medium (21.5%) staining for e-NOS. The median follow-up time was 42 months, during which 46 (28%) patients had a local recurrence or metastatic disease. A positive correlation of e-NOS with the hormone receptor status was found (P=0.031). However, no impact on survival rates was observed.

摘要

为评估内皮型一氧化氮合酶（e-NOS）在人类乳腺癌中的临床潜力，我们使用单克隆抗体对163例患者石蜡包埋的原发性乳腺癌组织进行了e-NOS免疫组织化学（IHC）染色。发现e-NOS表达与经典预后因素及生存率之间存在相关性。不到一半的患者处于绝经前（38.5%），61.5%为绝经后。肿瘤中位大小为2 cm；41.7%的患者有腋窝淋巴结受累。大多数肿瘤（84.1%）激素受体呈阳性。e-NOS染色阳性率为62%，大多数阳性肿瘤的e-NOS染色为弱阳性（32.5%）或中等强度阳性（21.5%）。中位随访时间为42个月，在此期间46例（28%）患者出现局部复发或转移性疾病。发现e-NOS与激素受体状态呈正相关（P = 0.031）。然而，未观察到对生存率有影响。