牛5号染色体5q1.3-q2.5的高分辨率辐射杂种图谱与人类12号染色体12q的比较

A high-resolution radiation hybrid map of bovine chromosome 5q1.3-q2.5 compared with human chromosome 12q.

作者信息

Mömke S, Drögemüller C, Distl O

机构信息

Institute for Animal Breeding and Genetics, University of Veterinary Medicine Hannover, Foundation, Bünteweg 17p, 30559 Hannover, Germany.

出版信息

Anim Genet. 2005 Jun;36(3):248-53. doi: 10.1111/j.1365-2052.2005.01287.x.

DOI:10.1111/j.1365-2052.2005.01287.x

PMID:15932408

Abstract

In this study we present a comprehensive 3000-rad radiation hybrid map on bovine chromosome 5 (BTA5) of a region between 12.8 and 74.0 cM according to the linkage map, which contains a quantitative trait loci for ovulation rate. We mapped 28 gene-associated sequence tagged site markers derived from sequences of bovine BAC clones and 10 microsatellite markers to the BTA5 region. In comparison with HSA12q, four blocks of conserved synteny were apparent showing three chromosomal breakpoints and two inversions in this segment of BTA5. Therefore, we have improved breakpoint resolution in the human-bovine comparative map, which enhances the determination of candidate genes underlying traits of interest mapped to BTA5.

摘要

在本研究中，我们根据连锁图谱展示了牛5号染色体（BTA5）上一段12.8至74.0厘摩区域的全面的3000拉德辐射杂种图谱，该区域包含一个排卵率的数量性状位点。我们将源自牛BAC克隆序列的28个基因相关序列标签位点标记和10个微卫星标记定位到BTA5区域。与人类12号染色体长臂（HSA12q）相比，明显存在四个保守同线性区段，显示出BTA5这一区段有三个染色体断点和两个倒位。因此，我们提高了人牛比较图谱中的断点分辨率，这增强了对定位到BTA5上的感兴趣性状潜在候选基因的确定。