Mu Jiao, He Zuo-yun, Yu Lu
Department of Cardiology, Xinqiao Hospital, Third Military Medical Univercity, PLA, Chongqing 400037, China.
Zhonghua Xin Xue Guan Bing Za Zhi. 2005 Apr;33(4):354-9.
To investigate the change and correlation of carbon monoxide/heme oxygenase and nitrogen monoxide/nitric oxide synthase system in atherosclerosis and the influence of the two systems on atherosclerotic progress.
The rabbits received 1% cholesterol diet (chol group, n = 8), or 1% cholesterol diet plus L-arginine (L-arg group, n = 8) or L-NAME (L-NAME group, n = 8) by drinking water, or 1% cholesterol diet plus heme-L-lysinate (Heme group, n = 8) or ZnPP-IX (ZnPP group, n = 8) by injection in abdominal cavity for 10 weeks.
Compared with those in control group, aortic NO production and expression of NOS decreased markedly; while CO production (P < 0.01) and HO-1 activity increased obviously in chol group. The aortic plaques area was (40.2 +/- 8.9)% in chol group. Compared with those in chol group, aortic areas [(26.6 +/- 9.2)%] reduced distinctly in heme group, aortic CO production and NOS activity increased obviously (P < 0.01) in L-arg group. However, compared with those in control group, HO-1 expression and CO production decreased markedly (P < 0.01) in heme group, while they were not different from those in chol group. Compared with those in chol group, aortic cNOS activity and NO production increased obviously and aortic plaques area [(28.1 +/- 7.7)%] greatly reduced (P < 0.01) in L-arg group. However, HO-1 expression and CO production of L-arg group decreased distinctly compared with those of control group, but they were similar to those of chol group. The aortic c-myc and c-fos expressions in both heme group and L-arg group reduced markedly compared with those in chol group, while they were similar to those in ZnPP and L-NAME group.
The reciprocal relationship between heme oxygenase/carbon monoxide and nitric oxide synthase/nitrogen monoxide system in atherosclerosis may play the inhibitory role against atherosclerotic lesion.
探讨一氧化碳/血红素加氧酶和一氧化氮/一氧化氮合酶系统在动脉粥样硬化中的变化及相关性,以及这两个系统对动脉粥样硬化进展的影响。
将家兔分为以下几组:给予1%胆固醇饮食(胆固醇组,n = 8),或通过饮水给予1%胆固醇饮食加L-精氨酸(L-精氨酸组,n = 8)或L-硝基精氨酸甲酯(L-NAME组,n = 8),或通过腹腔注射给予1%胆固醇饮食加血红素-L-赖氨酸盐(血红素组,n = 8)或锌原卟啉-IX(锌原卟啉组,n = 8),持续10周。
与对照组相比,胆固醇组主动脉一氧化氮生成及一氧化氮合酶表达明显降低;而一氧化碳生成(P < 0.01)及血红素加氧酶-1活性明显升高。胆固醇组主动脉斑块面积为(40.2±8.9)%。与胆固醇组相比,血红素组主动脉面积明显减小[(26.6±9.2)%],L-精氨酸组主动脉一氧化碳生成及一氧化氮合酶活性明显升高(P < 0.01)。然而,与对照组相比,血红素组血红素加氧酶-1表达及一氧化碳生成明显降低(P < 0.01),但与胆固醇组无差异。与胆固醇组相比,L-精氨酸组主动脉内皮型一氧化氮合酶活性及一氧化氮生成明显升高,主动脉斑块面积[(