Li Yong-qiang, Ma Hong, Dong Yu-gang
Department of Cardiology, First Affiliated Hospital of Zhong Shan University, Guangzhou 510080, China.
Zhonghua Xin Xue Guan Bing Za Zhi. 2005 May;33(5):395-8.
To evaluate the effects of Chlamydia pneumoniae infection on oxidative stress and the development of atherosclerosis in C57BL/6J mice.
Forty-eight C57BL/6J mice were divided into 4 groups including infection of CP and cholesterol diet, cholesterol diet, infection of CP and control. Atherosclerotic lesions were measured in the aortic root at 40 weeks after the primary infection. Production of superoxide was measured by lucigenin-enhanced chemiluminescence response and evaluated in situ with laser scanning confocal microscope.
Infected mice fed with an atherogenic diet developed significantly larger lesion areas compared with the single atherogenic diet mice (135 249 +/- 43 748 microm2 vs. 96 378 +/- 30 945 microm2, P < 0.05). Superoxide generation was higher in aortic arches of the infected mice or atherogenic diet mice compared with the control mice (1974.25 +/- 650.49, 701.00 +/- 105.16, 455.62 +/- 77.54 counts.mg(-1).min(-1) vs. 142.25 +/- 31.82 counts.mg(-1).min(-1), respectively, P < 0.001).
Chlamydia pneumoniae infection accelerates atherosclerotic lesion development in diet-induced hypercholesterolemic mice. Generation of reactive oxygen species may contribute to atherosclerotic development by Chlamydia pneumoniae infection.
评估肺炎衣原体感染对C57BL/6J小鼠氧化应激及动脉粥样硬化发展的影响。
将48只C57BL/6J小鼠分为4组，包括肺炎衣原体感染加胆固醇饮食组、胆固醇饮食组、肺炎衣原体感染组和对照组。在初次感染后40周测量主动脉根部的动脉粥样硬化病变。通过光泽精增强化学发光反应测量超氧化物的产生，并使用激光扫描共聚焦显微镜进行原位评估。
与单纯给予致动脉粥样硬化饮食的小鼠相比，给予致动脉粥样硬化饮食的感染小鼠的病变面积显著更大（135 249±43 748平方微米对96 378±30 945平方微米，P<0.05）。与对照小鼠相比，感染小鼠或给予致动脉粥样硬化饮食小鼠的主动脉弓中超氧化物生成更高（分别为1974.25±650.49、701.00±105.16、455.62±77.54计数·毫克⁻¹·分钟⁻¹对142.25±31.82计数·毫克⁻¹·分钟⁻¹，P<0.001）。
肺炎衣原体感染加速饮食诱导的高胆固醇血症小鼠动脉粥样硬化病变的发展。活性氧的产生可能通过肺炎衣原体感染促进动脉粥样硬化的发展。
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