Silberbach Maike, Hüser Andrea, Kalinowski Jörn, Pühler Alfred, Walter Britta, Krämer Reinhard, Burkovski Andreas
Institut für Biochemie, Universität zu Köln, Zülpicher Strasse 47, D-50674 Köln, Germany.
J Biotechnol. 2005 Oct 10;119(4):357-67. doi: 10.1016/j.jbiotec.2005.04.007.
Nitrogen is an essential component of nearly all of the complex macromolecules in a bacterial cell, e.g. proteins, nucleic acids, and cell wall components. Accordingly, most prokaryotes have developed elaborate control mechanisms to provide an optimal supply of nitrogen for cellular metabolism and to cope with situations of nitrogen limitation. In this communication, a global analysis of the Corynebacterium glutamicum nitrogen starvation response by transcriptional profiling using DNA microarrays is presented. Our results show that C. glutamicum reacts to nitrogen starvation with a rearrangement of the cellular transport capacity, changes in metabolic pathways concerning nitrogen assimilation and amino acid biosynthesis, and a decreased capacity for protein synthesis.
氮是细菌细胞中几乎所有复杂大分子的重要组成部分，例如蛋白质、核酸和细胞壁成分。因此，大多数原核生物已经发展出精细的调控机制，以确保为细胞代谢提供最佳的氮供应，并应对氮限制的情况。在本通讯中，我们通过DNA微阵列转录谱分析对谷氨酸棒杆菌的氮饥饿反应进行了全面分析。我们的结果表明，谷氨酸棒杆菌对氮饥饿的反应包括细胞转运能力的重新排列、与氮同化和氨基酸生物合成相关的代谢途径的变化，以及蛋白质合成能力的下降。
