Chatdarong Kaywalee, Rungsipipat Anudep, Axnér Eva, Linde Forsberg Catharina
Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences (SLU), Uppsala SE-75007, Sweden.
Theriogenology. 2005 Jul 1;64(1):12-29. doi: 10.1016/j.theriogenology.2004.10.018. Epub 2005 Jan 24.
The aims of this study were to characterize the hysterographic and histological features of the uteri and to perform immunohistochemistry with proliferating cell nuclear antigen (PCNA) in the cat endometrium at various stages of the reproductive cycle and after treatment with exogenous progestagen. Seventy-four female domestic cats submitted for routine ovariohysterectomy were categorized into six groups: inactive (n=20), follicular (n=9), luteal (n=18), and postpartum (n=12) stages of the reproductive cycle; cats given medroxyprogesterone acetate for estrus prevention (MPA group) (n=12); and cats with uterine pathological lesions (n=3). Hysterography was performed and the relation of the uterine and luminal shape in the hysterogram with the stage of the reproductive cycle as well as with any pathological conditions of the uterus was evaluated. The uteri and ovaries were thereafter surgically removed and sectioned for histological examination. The PCNA was used to demonstrate the expression of endometrial epithelial cell growth. The hysterographic appearance was found to differ between the six groups of cats. A straight uterine cavity was characteristic for cats in the inactive stage, whereas a wavy uterine cavity was characteristic for cats in the follicular stage. In the luteal stage, the luminal cavity of the uteri differed in shape with increasing progesterone concentration from straight to irregular wavy or coiled. The coil shaped uterine lumen seen in the MPA treated and pathological groups was considered also to be an expression of a progestagenic effect. Waviness and coiling of the uterine lumen was related to a proliferation of the endometrial glands, whereas irregular filling defects were indicative of endometrial cystic changes. This study is the first to demonstrate the expression of PCNA in the cat endometrium although no differences were found between the six groups of cats. The hysterographic appearance was found to differ according to stage of the reproductive cycle and pathological conditions. Thus, a normative hysterogram is now available for diagnosing the reproductive stage and uterine changes in cats developing endometrial hyperplasia with and without cystic changes.
本研究的目的是描述子宫的子宫造影和组织学特征，并在生殖周期的各个阶段以及用外源性孕激素治疗后，对猫子宫内膜中的增殖细胞核抗原（PCNA）进行免疫组织化学检测。将74只接受常规卵巢子宫切除术的雌性家猫分为六组：生殖周期的静止期（n = 20）、卵泡期（n = 9）、黄体期（n = 18）和产后（n = 12）阶段；给予醋酸甲羟孕酮预防发情的猫（MPA组）（n = 12）；以及患有子宫病理病变的猫（n = 3）。进行子宫造影，并评估子宫造影中子宫和管腔形状与生殖周期阶段以及子宫任何病理状况的关系。此后，手术切除子宫和卵巢并切片进行组织学检查。使用PCNA来证明子宫内膜上皮细胞生长的表达。发现六组猫的子宫造影表现有所不同。静止期的猫子宫腔呈直形，而卵泡期的猫子宫腔呈波浪形。在黄体期，随着孕酮浓度的增加，子宫的管腔形状从直形变为不规则波浪形或螺旋形。在MPA治疗组和病理组中看到的螺旋形子宫腔也被认为是孕激素作用的一种表现。子宫管腔的波浪状和螺旋状与子宫内膜腺体的增殖有关，而不规则的充盈缺损表明子宫内膜有囊性变化。本研究首次证明了PCNA在猫子宫内膜中的表达，尽管在六组猫之间未发现差异。发现子宫造影表现根据生殖周期阶段和病理状况而有所不同。因此，现在有了一个标准的子宫造影图，可用于诊断患有或未患有囊性变化的子宫内膜增生的猫的生殖阶段和子宫变化。
