Yang Xue-chao, Fan Ming-wen
Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School of Stomatology, Wuhan University, Wuhan 430079, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2005 May;40(3):244-7.
To isolate and cultivate human dental pulp stem cells (DPSCs).
Pulp tissue was removed from healthy young human teeth extracted for orthodontic purposes. The pulp was digested by Type I collagenase and dispase. Then single-cell suspensions were obtained by filter and cultivated. The clones were identified by expression of STRO-1. Under the conditions of inducement, clones were identified by activity of alkaline phosphatase (ALP), formation of mineralized nodule and expression of dentin sialoprotein (DSP), and by Oil Red-O dyeing and expressing of PPARr2.
The clones had positive expression of STRO-1. When stimulated to differentiation, these cells took on dramatically high activity of ALP, had the ability of mineralization and expressed DSP. These cells also had ability to trans-differentiate into adipocytes.
There are stem cells in human dental pulp tissues, which can be isolated and cultivated.
分离培养人牙髓干细胞（DPSCs）。
从因正畸拔除的健康年轻人牙齿中获取牙髓组织。用Ⅰ型胶原酶和 dispase 消化牙髓。然后通过过滤获得单细胞悬液并进行培养。通过 STRO-1 的表达鉴定克隆。在诱导条件下，通过碱性磷酸酶（ALP）活性、矿化结节形成和牙本质涎蛋白（DSP）表达，以及油红 O 染色和 PPARr2 表达鉴定克隆。
克隆具有 STRO-1 的阳性表达。当诱导分化时，这些细胞呈现出显著高的 ALP 活性，具有矿化能力并表达 DSP。这些细胞也具有向脂肪细胞转分化的能力。
人牙髓组织中存在干细胞，可进行分离培养。
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