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脉冲场凝胶电泳用于测量xrs - 6和CHO细胞系中的DNA双链断裂修复：某些条件下的DNA降解会干扰双链断裂重新连接的评估。

Pulsed-field gel electrophoresis in the measurement of DNA double-strand break repair in xrs-6 and CHO cell lines: DNA degradation under some conditions interferes with the assessment of double-strand break rejoining.

作者信息

Whitaker S J, McMillan T J

机构信息

Radiotherapy Research Unit, Institute of Cancer Research, Sutton, Surrey, United Kingdom.

出版信息

Radiat Res. 1992 Jun;130(3):389-92.

PMID:1594768
Abstract

Pulsed-field gel electrophoresis (PFGE) is a sensitive assay for DNA double-strand breaks (DSBs). The application of PFGE to the measurement of DSB rejoining requires attention to conditions after irradiation. When cells are irradiated and incubated while encapsulated in agarose, DNA DSB rejoining appears incomplete or absent due to DNA degradation artifacts. Using PFGE we have measured the DSB rejoining defect in xrs-6 cells and shown how DNA degradation can affect the results.

摘要

脉冲场凝胶电泳（PFGE）是一种检测DNA双链断裂（DSB）的灵敏方法。将PFGE应用于DSB修复的测量时，需要注意辐照后的条件。当细胞在琼脂糖包封状态下接受辐照并孵育时，由于DNA降解假象，DNA DSB修复似乎不完整或不存在。我们使用PFGE测量了xrs-6细胞中的DSB修复缺陷，并展示了DNA降解如何影响结果。

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The use of caspase inhibitors in pulsed-field gel electrophoresis may improve the estimation of radiation-induced DNA repair and apoptosis.
在脉冲场凝胶电泳中使用半胱天冬酶抑制剂可能会提高对辐射诱导的 DNA 修复和细胞凋亡的估计。
Radiat Oncol. 2011 Jan 15;6:6. doi: 10.1186/1748-717X-6-6.