Wu Xia, Sun Shuna, Yang Jinghe, Wang Minqin, Liu Liyan, Guo Changying
Key Laboratory of Colloid and Interface Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, Shandong, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2005 Dec;62(4-5):896-901. doi: 10.1016/j.saa.2005.03.022. Epub 2005 Jun 13.
At pH 9.75, the resonance light scattering (RLS) intensity of OA-Eu3+ system is greatly enhanced by nucleic acid. Based on this phenomenon, a new quantitative method for nucleic acid in aqueous solution has been developed. Under the optimum condition, the enhanced RLS is proportional to the concentration of nucleic acid in the range of 1.0x10(-9) to 1.0x10(-6)g/ml for herring sperm DNA, 8.0x10(-10) to 1.0x10(-6) g/ml for calf thymus DNA and 1.0x10(-9) to 1.0x10(-6) g/ml for yeast RNA, and their detection limits are 0.020, 0.011 and 0.010 ng/ml, respectively. Synthetic samples and actual samples were satisfactorily determined. In addition, the interaction mechanism between nucleic acid and OA-Eu3+ is also investigated.
在pH 9.75时,核酸可极大增强OA-Eu3+体系的共振光散射(RLS)强度。基于此现象,已开发出一种测定水溶液中核酸的新定量方法。在最佳条件下,对于鲱鱼精DNA,增强的RLS在1.0×10(-9)至1.0×10(-6)g/ml范围内与核酸浓度成正比;对于小牛胸腺DNA,在8.0×10(-10)至1.0×10(-6) g/ml范围内成正比;对于酵母RNA,在1.0×10(-9)至1.0×10(-6) g/ml范围内成正比,它们的检测限分别为0.020、0.011和0.010 ng/ml。合成样品和实际样品均得到了满意的测定结果。此外,还研究了核酸与OA-Eu3+之间的相互作用机理。