Ansorena D, Raes K, De Smet S, Demeyer D
Department of Food Science and Food Technology, University of Navarra, Irunlarrea s/n, 31008 Pamplona, Spain.
Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet. 2001;66(3b):365-72.
A method for the analysis of trans fatty acids is described in this paper. Separation of trans isomers from the corresponding cis was based on the different facility with which the double bonds in the alkyl chains of both types of fatty acids form polar complexes with silver, and it was carried out by Thin Layer Chromatography (TLC), using silver nitrate impregnated plates. Further separation, identification and quantification of the individual C18:1 isomers, analysed as methyl esters, was carried out by Gas Chromatography (GC). The determination of the content of the isomer t11-C18:1 in ruminant tissues can contribute to try to elucidate the metabolic pathway formation of conjugated linoleic acid (CLA), shown as a fatty acid of beneficial health effects. Furthermore, the analysis of trans fatty acids in other dietary foods seems to be interesting due to their influence on the modification of the serum lipids profile and the role of this type of compounds in human health.
本文描述了一种反式脂肪酸的分析方法。反式异构体与相应顺式异构体的分离基于两种脂肪酸烷基链中的双键与银形成极性络合物的难易程度不同，通过使用硝酸银浸渍板的薄层色谱法（TLC）进行分离。作为甲酯分析的各个C18:1异构体的进一步分离、鉴定和定量通过气相色谱法（GC）进行。反刍动物组织中异构体t11-C18:1含量的测定有助于试图阐明共轭亚油酸（CLA）的代谢途径形成，CLA是一种具有有益健康作用的脂肪酸。此外，由于其他膳食食品中的反式脂肪酸对血清脂质谱的改变有影响以及这类化合物在人类健康中的作用，对其进行分析似乎很有意义。
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