Libros-Ziv Pazit, Villalobo Eduardo, Mirelman David
Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel.
Exp Parasitol. 2005 Jul;110(3):276-9. doi: 10.1016/j.exppara.2005.02.018. Epub 2005 Apr 1.
Entamoeba histolytica is a phagocytic cell with numerous vesicles of different sizes and shapes but without a well-defined Golgi apparatus. Despite this, genes implied in membrane trafficking have been identified in the genome of this parasite. One of these genes is homologous to the N-ethylmaleimide sensitive fusion factor (NSF), whose protein has been shown to play an important role in vesicle fusion in other eukaryotic cells. In this report, we investigated the NSF homologue gene from a pathogenic E. histolytica, characterized its protein product and two of its activities, ATPase and in vitro intra-Golgi transport. The finding of an active NSF protein in E. histolytica indicates that a simple or primordial Golgi apparatus probably exists in this microorganism, as has been proposed by others.
溶组织内阿米巴是一种吞噬细胞，具有许多大小和形状各异的囊泡，但没有明确的高尔基体。尽管如此，在这种寄生虫的基因组中已经鉴定出了与膜运输相关的基因。其中一个基因与N - 乙基马来酰亚胺敏感融合因子（NSF）同源，其蛋白质已被证明在其他真核细胞的囊泡融合中起重要作用。在本报告中，我们研究了来自致病性溶组织内阿米巴的NSF同源基因，对其蛋白质产物及其两种活性（ATP酶活性和体外高尔基体内运输活性）进行了表征。在溶组织内阿米巴中发现有活性的NSF蛋白表明，正如其他人所提出的那样，这种微生物中可能存在简单或原始的高尔基体。
