松弛素下调MDA-MB-231人乳腺癌细胞中的钙结合蛋白S100A4。

Relaxin downregulates the calcium binding protein S100A4 in MDA-MB-231 human breast cancer cells.

作者信息

Radestock Yvonne, Hoang-Vu Cuong, Hombach-Klonisch Sabine

机构信息

Department of Human Anatomy and Cell Sciences, University of Manitoba, Faculty of Medicine, 130 Basic Medical Sciences, Winnipeg, MB, R3E 0W3, Canada.

出版信息

Ann N Y Acad Sci. 2005 May;1041:462-9. doi: 10.1196/annals.1282.070.

DOI:10.1196/annals.1282.070

PMID:15956747

Abstract

Expressed in the human breast and in human breast cancer tissues, the heterodimeric peptide hormone relaxin is involved in extracellular matrix turnover. To investigate the role of relaxin in estrogen receptor-alpha negative human breast cancer cells, we established transfectants of the human MDA-MB-231 breast cancer cell line stably overexpressing H2-relaxin (MDA-MB-231/pIRES-EGFP-H2). These transfectants produced and secreted functional relaxin. Our investigations revealed downregulation of mRNA and protein of the calcium binding protein S100A4 (metastasin) in MDA-MB-231/pIRES-EGFP-H2 transfectants, indicating a distinct and novel role for relaxin in estrogen receptor-alpha negative human breast cancer cells.

摘要

异二聚体肽激素松弛素在人乳腺及人乳腺癌组织中表达，参与细胞外基质周转。为研究松弛素在雌激素受体α阴性人乳腺癌细胞中的作用，我们建立了稳定过表达H2 - 松弛素的人MDA - MB - 231乳腺癌细胞系转染子（MDA - MB - 231/pIRES - EGFP - H2）。这些转染子产生并分泌有功能的松弛素。我们的研究揭示，MDA - MB - 231/pIRES - EGFP - H2转染子中钙结合蛋白S100A4（转移抑制蛋白）的mRNA和蛋白质表达下调，表明松弛素在雌激素受体α阴性人乳腺癌细胞中具有独特的新作用。