Gene discovery by ribozyme and siRNA libraries.

作者信息

Akashi Hideo, Matsumoto Sahohime, Taira Kazunari

机构信息

Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Tokyo 113-8656, Japan.

出版信息

Nat Rev Mol Cell Biol. 2005 May;6(5):413-22. doi: 10.1038/nrm1646.

DOI:10.1038/nrm1646

PMID:15956980

Abstract

Catalytic RNAs, also known as ribozymes, can be engineered to optimize their activities in the intracellular environment. The introduction of a library of active ribozymes into cells, and the subsequent screening for phenotypic changes, allows the rapid identification of gene function. For the determination of gene function, ribozyme technology complements another RNA-based tool that is based on libraries of small interfering RNAs.

摘要