Gershenzon J, McCaskill D, Rajaonarivony J I, Mihaliak C, Karp F, Croteau R
Institute of Biological Chemistry, Washington State University, Pullman 99164-6340.
Anal Biochem. 1992 Jan;200(1):130-8. doi: 10.1016/0003-2697(92)90288-i.
The natural products that accumulate in or exude from plant glandular trichomes are biosynthesized by secretory cells located at the apex of the trichome. To investigate the formation of glandular trichome constituents in several species of mints (Lamiaceae), a new procedure was developed for isolating large numbers of highly purified secretory cells. In this method, the leaf surface is gently abraded with glass beads in a way that fragments the glandular trichomes and yields clusters of intact secretory cells. The isolated, intact secretory cells and cell-free preparations derived from them are very active in monoterpene biosynthesis and provide useful starting materials for the purification of several key enzymes of monoterpene metabolism. The procedure described is adaptable to a broad range of plant species and should find wide application in the preparation of whole cell and cell-free systems for biosynthetic studies of plant natural products found in glandular trichomes.
植物腺毛中积累或分泌出的天然产物是由位于腺毛顶端的分泌细胞生物合成的。为了研究几种薄荷属植物（唇形科）中腺毛成分的形成，开发了一种新方法来分离大量高度纯化的分泌细胞。在该方法中，用玻璃珠轻轻摩擦叶片表面，使腺毛破碎并产生完整分泌细胞簇。分离得到的完整分泌细胞及其衍生的无细胞制剂在单萜生物合成中非常活跃，为纯化单萜代谢的几种关键酶提供了有用的起始材料。所述方法适用于广泛的植物物种，在制备用于研究腺毛中植物天然产物生物合成的全细胞和无细胞系统方面应具有广泛的应用。
