使用硝酸纤维素膜固定化乳腺催乳素受体的血清催乳素放射受体测定法。

Radioreceptor assay of serum prolactin using nitrocellulose membrane-immobilized mammary prolactin receptor.

作者信息

Suzuki M, Kohmoto K, Sakai S

机构信息

Department of Animal Breeding, Faculty of Agriculture, University of Tokyo, Japan.

出版信息

Anal Biochem. 1992 Jan;200(1):42-6. doi: 10.1016/0003-2697(92)90274-b.

DOI:10.1016/0003-2697(92)90274-b

PMID:1595899

Abstract

Triton-solubilized rabbit mammary prolactin (PRL) receptor was purified by concanavalin A-agarose chromatography and immobilized on a nitrocellulose membrane. Using the membrane-bound receptor and bovine serum, the serum level of PRL was determined by radioreceptor assay (RRA). The displacement curve, obtained by serial dilutions of the serum, was parallel to that obtained in the range of 0.4 and 20 ng/ml of standard bovine PRL. Serum could be included in concentrations up to 16% of the assay buffer and, thus, the detection limit of serum PRL was about 2.5 ng/ml. PRL in the serum was determined by radioimmunoassay (RIA). The ratio of the RIA and RRA estimates of PRL was 0.99 (r = +0.99, n = 55). The gel-filtration profile of serum PRL was identical to that obtained by RIA. It was concluded that membrane-bound receptor can be used for the determination of serum PRL.

摘要

用伴刀豆球蛋白A - 琼脂糖层析法纯化了经曲拉通增溶的兔乳腺催乳素（PRL）受体，并将其固定在硝酸纤维素膜上。使用膜结合受体和牛血清，通过放射受体分析（RRA）测定血清中PRL的水平。通过血清系列稀释得到的置换曲线与在0.4至20 ng/ml标准牛PRL范围内得到的曲线平行。血清可按高达测定缓冲液16%的浓度加入，因此，血清PRL的检测限约为2.5 ng/ml。通过放射免疫分析（RIA）测定血清中的PRL。PRL的RIA和RRA估计值之比为0.99（r = +0.99，n = 55）。血清PRL的凝胶过滤图谱与通过RIA得到的图谱相同。得出的结论是，膜结合受体可用于测定血清PRL。