Tibary A, Anouassi A, Khatir H
Department of Veterinary Clinical Sciences and Center for Reproductive Biology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-6610, USA.
Theriogenology. 2005 Aug;64(3):618-38. doi: 10.1016/j.theriogenology.2005.05.016.
Recent advances in reproductive biotechnologies in small ruminants include improvement of methods for in vitro production of embryos and attempts at spermatogonial stem cell transplantation. In vitro production of embryos by IVM/IVF, intra-cytoplasmic sperm injection (ICSI), or nuclear transfer (NT) has been made possible by improvements in oocyte collection and maturation techniques, and early embryo culture systems. However, in vitro embryo production still is not very efficient due to several limiting factors affecting the outcome of each step of the process. This paper discusses factors affecting in vitro embryo production in small ruminants and camelids, as well as preliminary results with the technique of spermatogonial stem cell transplantation.
小型反刍动物生殖生物技术的最新进展包括胚胎体外生产方法的改进以及精原干细胞移植的尝试。通过改进卵母细胞采集和成熟技术以及早期胚胎培养系统,借助体外成熟/体外受精(IVM/IVF)、胞浆内精子注射(ICSI)或核移植(NT)进行胚胎体外生产已成为可能。然而,由于影响该过程每个步骤结果的几个限制因素,体外胚胎生产效率仍然不是很高。本文讨论了影响小型反刍动物和骆驼科动物体外胚胎生产的因素,以及精原干细胞移植技术的初步结果。