Takahashi Hideo, Saito Hiuga, Ikeda Yonosuke
Insitute of Applied Microbiology, University of Tokyo, Tokyo.
J Gen Appl Microbiol. 2004 Dec;50(6):363-70.
The conserved portion in bacterial ribosomal RNA was studied by the DNA-RNA hybridization method. The hybridization percentages were as follows: Bacillus subtilis DNA and B. subtilis 23S rRNA, 0.16; Escherichia coli DNA and E. coli 23S rRNA, 0.15; B. subtilis DNA and E. coli 23S rRNA, 0.03; E. coli DNA and B. subtilis 23S rRNA, 0.04. The RNA's extracted from the heterologous hybrids could be rehybridized with DNA's of B. subtilis and E. coli. The average chain lengths of the RNA's were estimated by sucrose density gradient centrifugation and Sephadex gel filtration. The results suggested that the size might be larger than 30 nucleotides. Nucleotide compositions of the RNA's in the hybrids were also studied. Both RNA's contained higher molar percentages of guanylic acid and cytidylic acid than the whole rRNA's.
采用DNA-RNA杂交法研究了细菌核糖体RNA中的保守部分。杂交百分率如下：枯草芽孢杆菌DNA与枯草芽孢杆菌23S rRNA，0.16；大肠杆菌DNA与大肠杆菌23S rRNA，0.15；枯草芽孢杆菌DNA与大肠杆菌23S rRNA，0.03；大肠杆菌DNA与枯草芽孢杆菌23S rRNA，0.04。从异源杂交体中提取的RNA可与枯草芽孢杆菌和大肠杆菌的DNA再次杂交。通过蔗糖密度梯度离心和葡聚糖凝胶过滤法估计了RNA的平均链长。结果表明其大小可能大于30个核苷酸。还研究了杂交体中RNA的核苷酸组成。两种RNA中鸟苷酸和胞苷酸的摩尔百分比均高于整个rRNA。
