Liu Shuang-Jiang, Lütke-Eversloh Tina, Steinbüchel Alexander
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Sheng Wu Gong Cheng Xue Bao. 2003 Mar;19(2):195-9.
Polythioesters newly emerged as a type of novel polymer and they have showed great potential for application in industries. In this study, genes of butyrate kinase (buk) and phosphotransbutyrylase (ptb) from Clostridium acetobutylicum, and poly (3-hydroxybutyrate) (PHB) synthase gene from Thiocapsa pfennigii were used for construction of a metabolic pathway to synthesize the polythioesters. When 3-mercaptopropionate and 3-hydroxybutyrate were fed, poly (3-mercaptopropoinate) [poly (3MP)] and poly(3-mercaptopropionate-co-3-hydroxybutyrate) [poly(3MP-co-3HB)] were synthesized by recombinant Escherichia coli JM109 (pBPP1) harboring the constructed metabolic pathway. Results indicated clearly that all these genes are necessary for the synthesis of poly(3MP) and poly(3MP-co-3HB).
聚硫酯作为一种新型聚合物新近出现，并且它们在工业应用中显示出了巨大潜力。在本研究中，丙酮丁醇梭菌的丁酸激酶（buk）和磷酸转丁酰酶（ptb）基因，以及嗜硫小红卵菌的聚（3-羟基丁酸酯）（PHB）合酶基因被用于构建一条合成聚硫酯的代谢途径。当加入3-巯基丙酸酯和3-羟基丁酸酯时，含有构建好的代谢途径的重组大肠杆菌JM109（pBPP1）合成了聚（3-巯基丙酸酯）[聚（3MP）]和聚（3-巯基丙酸酯-共-3-羟基丁酸酯）[聚（3MP-共-3HB）]。结果清楚地表明，所有这些基因对于聚（3MP）和聚（3MP-共-3HB）的合成都是必需的。
