[白毛茛中生物碱的提取及高效液相色谱分析]

[Extraction and HPLC analysis of alkaloids in goldenseal].

作者信息

Weber Holly A, Joseph Maureen

机构信息

Midwest Research Institute 425 Volker Blvd Kansas City, MO 64110, USA.

出版信息

Sheng Wu Gong Cheng Xue Bao. 2004 Mar;20(2):306-8.

PMID:15969129

Abstract

An ambient extraction of goldenseal root powder followed by HPLC analysis of the alkaloids on a Zorbax Rapid Resolution Eclipse XDB-C18 column provides an accurate method for the determination of key alkaloids in goldenseal, including berberine and hydrastine. The extraction and HPLC analysis can be applied to several other alkaloids, including canadine, hydrastinine, and palmatine, and may be applicable to other berberine-containing plant roots. The Rapid Resolution Eclipse XDB-C18 column is used for an isocratic separation with high resolution of all componentsin under 15 minutes.

摘要

对白毛茛根粉进行环境萃取，然后在Zorbax Rapid Resolution Eclipse XDB-C18柱上对生物碱进行HPLC分析，为测定白毛茛中的关键生物碱（包括小檗碱和白毛茛碱）提供了一种准确的方法。该萃取和HPLC分析可应用于其他几种生物碱，包括加拿大黄连碱、白毛茛宁和巴马汀，并且可能适用于其他含小檗碱的植物根。Rapid Resolution Eclipse XDB-C18柱用于等度分离，可在15分钟内对所有成分进行高分辨率分离。