Vercruysse Lieselot, Smagghe Guy, Herregods Griet, Van Camp John
Laboratory of Agrozoology, Department of Crop Protection, and Laboratory of Food Science, Faculty of Bioscience Engineering, Ghent University, Belgium.
J Agric Food Chem. 2005 Jun 29;53(13):5207-11. doi: 10.1021/jf050337q.
In this paper, ACE inhibitory activity in insect protein hydrolyzed by various enzymes (gastrointestinal proteases, alcalase, and thermolysin) is reported for the first time. Four insects of different insect orders were tested: Spodoptera littoralis (Lepidoptera), Bombyx mori (Lepidoptera), Schistocerca gregaria (Orthoptera), and Bombus terrestris (Hymenoptera). ACE inhibitory activity was measured by two different methods: a spectrophotometric method using FAPGG (2-furanacryloyl-phenylalanyl-glycyl-glycine) as substrate, and an HPLC method using dansyltriglycine (DTG) as substrate. Hydrolysis of the insect protein resulted in an increased ACE inhibitory activity. Overall, the highest ACE inhibitory activity was obtained after gastrointestinal digestion. These results suggest a role for insect protein as antihypertensive component in functional foods and nutraceuticals. Furthermore, the ACE inhibitory activity differed according to the method used. As a consequence, there is a need to standardize methodologies to evaluate ACE inhibitory activity.
本文首次报道了不同酶（胃肠蛋白酶、碱性蛋白酶和嗜热菌蛋白酶）水解昆虫蛋白后的ACE抑制活性。测试了四种不同昆虫目的昆虫：草地贪夜蛾（鳞翅目）、家蚕（鳞翅目）、沙漠蝗（直翅目）和地熊蜂（膜翅目）。采用两种不同方法测定ACE抑制活性：一种是以FAPGG（2-呋喃丙烯酰-苯丙氨酰-甘氨酰-甘氨酸）为底物的分光光度法，另一种是以丹磺酰三甘氨酸（DTG）为底物的HPLC法。昆虫蛋白水解后ACE抑制活性增强。总体而言，胃肠消化后获得的ACE抑制活性最高。这些结果表明昆虫蛋白在功能性食品和营养保健品中作为降压成分发挥作用。此外，ACE抑制活性因所用方法而异。因此，需要规范评估ACE抑制活性的方法。
