Ricci-Silva Maria Esther, Konno Katsuhiro, Faria Fernanda, Rádis-Baptista Gandhi, Fontes Wagner, Stöcklin Reto, Michalet Sophie, Yamane Tetsuo, Chudzinski-Tavassi Ana Marisa
Laboratory of Biochemistry and Biophysics, Butantan Institute, São Paulo, Brazil.
OMICS. 2005 Summer;9(2):194-208. doi: 10.1089/omi.2005.9.194.
The salivary complex of leeches contains many components able to modulate physiological mechanisms, such as coagulation and fibrinolysis, and it is composed by the salivary glands and proboscis, encompassing two different proteomes. The bidimensional electrophoretic pattern of the salivary complex from the Haementeria depressa leech revealed a total of 352 spots, 103 in common with the muscular tissue and 249 exclusive from the salivary complex as detected by silver staining; these spots showed isoelectric points from 3.5 to 9.5 and covered an apparent molecular weight range from 10 to 105 kDa. The following isoforms of proteins were identified by mass spectrometry analysis: antiplatelet protein, myohemerythrin and carbonic anhydrase. Since the leeches were not fed for about 2-3 months to stimulate the secretion of proteins that facilitates the blood metabolism, these most abundant proteins in the salivary complex excised from leeches, are expected to play a role during feeding and might have some anti-hemostatic properties. Furthermore, by zymography, a gelatinolytic and a fibrinolytic protein were identified.
水蛭的唾液复合体包含许多能够调节生理机制的成分，如凝血和纤维蛋白溶解，它由唾液腺和吻组成，包含两种不同的蛋白质组。来自Haementeria depressa水蛭唾液复合体的双向电泳图谱显示共有352个斑点，其中103个与肌肉组织共有，通过银染检测到249个是唾液复合体独有的；这些斑点的等电点在3.5至9.5之间，表观分子量范围为10至105 kDa。通过质谱分析鉴定出以下蛋白质同工型：抗血小板蛋白、肌红血球素和碳酸酐酶。由于水蛭约2 - 3个月未进食以刺激促进血液代谢的蛋白质分泌，从水蛭中切除的唾液复合体中这些最丰富的蛋白质预计在进食过程中发挥作用，并且可能具有一些抗止血特性。此外，通过酶谱法鉴定出一种明胶溶解蛋白和一种纤维蛋白溶解蛋白。
