重组狂犬病病毒疫苗株SAD-l16经脑内接种幼年小鼠后，会引发严重的脑炎，并伴有广泛的神经元凋亡。
Recombinant rabies virus vaccine strain SAD-l16 inoculated intracerebrally in young mice produces a severe encephalitis with extensive neuronal apoptosis.
作者信息
Rasalingam Pamini, Rossiter John P, Jackson Alan C
机构信息
Department of Microbiology and Immunology, Queen's University, Kingston, Ontario.
出版信息
Can J Vet Res. 2005 Apr;69(2):100-5.
Abstract
Seven-day-old ICR mice were infected by intracerebral inoculation with recombinant rabies virus vaccine strain SAD-L16. Infected mice developed severe and fatal encephalitis with rabies virus-infected neurons in widespread regions of the brain. There was extensive neuronal death with predominant features of apoptosis, as assessed by light and electron microscopy, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) staining, and immunohistochemical staining for activated caspase-3. Although SAD-L16 is a neuroattenuated rabies virus, it is fully capable of spreading efficiently and inducing widespread neuronal apoptosis in the immature mouse brain.
摘要
7日龄的ICR小鼠通过脑内接种重组狂犬病病毒疫苗株SAD-L16进行感染。受感染的小鼠发生了严重的致命性脑炎，大脑广泛区域出现狂犬病病毒感染的神经元。通过光学显微镜和电子显微镜、末端脱氧核苷酸转移酶介导的dUTP-生物素缺口末端标记（TUNEL）染色以及活化的半胱天冬酶-3的免疫组织化学染色评估，存在广泛的神经元死亡，主要特征为凋亡。尽管SAD-L16是一种神经减毒狂犬病病毒，但它完全能够在未成熟的小鼠大脑中有效传播并诱导广泛的神经元凋亡。
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本文引用的文献
1
Comparative pathogenesis of the SAD-L16 strain of rabies virus and a mutant modifying the dynein light chain binding site of the rabies virus phosphoprotein in young mice.
Virus Res. 2005 Jul;111(1):55-60. doi: 10.1016/j.virusres.2005.03.010.
2
The application of reverse genetics technology in the study of rabies virus (RV) pathogenesis and for the development of novel RV vaccines.
J Neurovirol. 2005 Feb;11(1):76-81. doi: 10.1080/13550280590900436.
3
Intracerebral injections and the growth of viruses in the mouse brain.
Br J Exp Pathol. 1960 Feb;41(1):52-9.
4
Apoptosis and caspases in neurodegenerative diseases.
N Engl J Med. 2003 Apr 3;348(14):1365-75. doi: 10.1056/NEJMra022366.
5
Ultrastructure of retinal ganglion cell death after axotomy in chick embryos.
J Comp Neurol. 2002 Nov 25;453(4):361-71. doi: 10.1002/cne.10411.
6
Extensive attenuation of rabies virus by simultaneously modifying the dynein light chain binding site in the P protein and replacing Arg333 in the G protein.
J Virol. 2001 Dec;75(23):11496-502. doi: 10.1128/JVI.75.23.11496-11502.2001.
7
Programmed cell death in virus infections of the nervous system.
Curr Top Microbiol Immunol. 2001;253:95-119. doi: 10.1007/978-3-662-10356-2_5.
8
Viral mechanisms of immune evasion.
Trends Microbiol. 2000 Sep;8(9):410-8. doi: 10.1016/s0966-842x(00)01830-8.
9
Altruistic cell suicide and the specialized case of the virus-infected nervous system.
Trends Neurosci. 2000 Jul;23(7):284-90. doi: 10.1016/s0166-2236(00)01591-5.
10
An update on safety studies of SAD B19 rabies virus vaccine in target and non-target species.
Epidemiol Infect. 1999 Aug;123(1):165-75. doi: 10.1017/s0950268899002666.
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