由面包酵母中的α-葡萄糖苷酶合成对苯二酚-α-葡萄糖苷

Synthesis of hydroquinone-alpha-glucoside by alpha-glucosidase from baker's yeast.

作者信息

Prodanović Radivoje M, Milosavić Nenad B, Sladić Dusan, Velicković Tanja Cirković, Vujcić Zoran

机构信息

Department of Biochemistry, Faculty of Chemistry, University of Belgrade, Studentski trg 12, 11000, Belgrade, Serbia and Montenegro.

出版信息

Biotechnol Lett. 2005 Apr;27(8):551-4. doi: 10.1007/s10529-005-2880-9.

DOI:10.1007/s10529-005-2880-9

PMID:15973488

Abstract

Hydroquinone-alpha-glucoside was synthesised from hydroquinone and maltose as glucosyl donor by transglucosylation in a water system with alpha-glucosidase from baker's yeast. Only one phenolic -OH group was alpha-anomer-selectively glucosylated. The optimum conditions for transglucosylation reaction were at 30 degrees C for 20 h with 50 mM: hydroquinone and 1.5 M: maltose in 100 mM: sodium citrate/phosphate buffer at pH 5.5. The glucoside was obtained at 0.6 mg/ml with a 4.6% molar yield with respect to hydroquinone.

摘要

对苯二酚-α-葡萄糖苷是在水体系中，以面包酵母的α-葡萄糖苷酶为催化剂，由对苯二酚和作为葡萄糖基供体的麦芽糖通过转糖基化反应合成的。只有一个酚羟基被α-异头物选择性地葡萄糖基化。转糖基化反应的最佳条件是在30℃下反应20小时，对苯二酚浓度为50 mM，麦芽糖浓度为1.5 M，在pH 5.5的100 mM柠檬酸钠/磷酸盐缓冲液中进行。相对于对苯二酚，葡萄糖苷的产量为0.6 mg/ml，摩尔产率为4.6%。