De Castro Cristina, Molinaro Antonio, Lanzetta Rosa, Holst Otto, Parrilli Michelangelo
Dipartimento di Chimica Organica e Biochimica, Università degli studi di Napoli Federico II, I-80126 Napoli, Italy.
Carbohydr Res. 2005 Aug 15;340(11):1802-7. doi: 10.1016/j.carres.2005.05.010.
From the lipopolysaccharide (LPS) fraction of the plant-pathogenic bacterium Burkholderia caryophylli, the linkage between O-specific caryan and core region was characterised. The LPS fraction was first treated with 48% aqueous HF at 4 degrees C and successively with 1% acetic acid at 100 degrees C. A main oligosaccharide representing the carbohydrate backbone of the core region and a portion of the caryan (three unit of caryose) was isolated by high-performance anion-exchange chromatography. Compositional and methylation analyses, matrix-assisted laser desorption/ionisation mass spectrometry and 2D NMR spectroscopy identified the structure: [carbohydrate structure: see text]. The above residues are alpha-linked pyranose rings, if not stated otherwise. Hep is L-glycero-D-manno-heptose, Car is 4,8-cyclo-3,9-dideoxy-L-erythro-D-ido-nonose and Kdo is 3-deoxy-D-manno-oct-2-ulosonic acid. This finding indicates that QuiNAc residue is the primer monosaccharide, which connects the core oligosaccharide to caryan O-chain.
从植物致病细菌石竹伯克霍尔德氏菌（Burkholderia caryophylli）的脂多糖（LPS）组分中，对O-特异性石竹糖（caryan）与核心区域之间的连接进行了表征。LPS组分首先在4℃下用48%的氢氟酸水溶液处理，然后在100℃下依次用1%的乙酸处理。通过高效阴离子交换色谱法分离出一种代表核心区域碳水化合物主链和一部分石竹糖（三个石竹糖单元）的主要寡糖。组成分析、甲基化分析、基质辅助激光解吸/电离质谱和二维核磁共振光谱确定了其结构：[碳水化合物结构：见正文]。除非另有说明，上述残基均为α-连接的吡喃糖环。Hep是L-甘油-D-甘露庚糖，Car是4,8-环-3,9-二脱氧-L-赤藓糖-D-艾杜糖壬糖，Kdo是3-脱氧-D-甘露糖辛酸。这一发现表明，奎尼酰基（QuiNAc）残基是将核心寡糖与石竹糖O链连接的起始单糖。
