成釉细胞瘤中釉原蛋白基因（AMGX、AMGY）表达的分析。

Analysis of amelogenin gene (AMGX, AMGY) expression in ameloblastoma.

作者信息

Tsujigiwa Hidetsugu, Nagatsuka Hitoshi, Han Phuu Pwint, Gunduz Mehmet, Siar Chong Huat, Oida Shinichiro, Nagai Noriyuki

机构信息

Department of Oral Pathology and Medicine, Graduate School of Medicine and Dentistry, Okayama University, Japan.

出版信息

Oral Oncol. 2005 Sep;41(8):843-50. doi: 10.1016/j.oraloncology.2005.04.005.

DOI:10.1016/j.oraloncology.2005.04.005

PMID:15979380

Abstract

Although the amelogenin gene is expressed in ameloblastoma, the precise expression pattern of X and Y amelogenin genes (AMGX, AMGY) in this tumor has not yet been identified. In this study, we analyzed amelogenin gene expression in 19 samples (9 male, 10 female) of oral ameloblastomas by RT-PCR and detect the chromosomal origin of amelogenin mRNA by restriction enzyme digestion of the RT-PCR product. All tumor samples expressed amelogenin mRNA. We could detect increased level of AMGY expression in all male samples, higher than that of AMEX. It is an interesting finding as in normal male tooth development, the expression of AMGY is very much lower than that of AMGX. We postulate that epigenetic change of sex chromosomes may have some correlations with tumorigenesis of ameloblastoma. We also discuss the other possible mechanisms and points for future studies on this change in expression pattern.

摘要

尽管釉原蛋白基因在成釉细胞瘤中表达，但该肿瘤中X和Y釉原蛋白基因（AMGX、AMGY）的确切表达模式尚未明确。在本研究中，我们通过逆转录聚合酶链反应（RT-PCR）分析了19例口腔成釉细胞瘤样本（9例男性，10例女性）中釉原蛋白基因的表达情况，并通过对RT-PCR产物进行限制性酶切来检测釉原蛋白mRNA的染色体来源。所有肿瘤样本均表达釉原蛋白mRNA。我们在所有男性样本中检测到AMGY表达水平升高，高于AMEX。这是一个有趣的发现，因为在正常男性牙齿发育过程中，AMGY的表达远低于AMGX。我们推测性染色体的表观遗传变化可能与成釉细胞瘤的肿瘤发生存在某种关联。我们还讨论了其他可能的机制以及关于这种表达模式变化未来研究的要点。