Cooper Stephen
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109-0620, USA.
Brain Res Brain Res Protoc. 2005 Sep;15(3):115-8. doi: 10.1016/j.brainresprot.2005.05.002.
Serum starvation of astrocytes for a period of time followed by refeeding has been proposed as a method to produce synchronized astrocytes. Here, it is proposed that the method neither synchronizes cells nor satisfies rigorous criteria for cell synchronization. The proposed non-selective, whole-culture synchronization protocol cannot, in theory, synchronize cells. The cells produced by the proposed serum starvation/refeeding protocol do not reflect the properties of any particular cell during the cell cycle. Cells produced by the published protocol will have a wide distribution of cell sizes, and therefore, the cells produced by starvation/refeeding will not model cells of any specific age during the division cycle. Thus, the proposed protocol will not produce a synchronized culture.
一段时间的星形胶质细胞血清饥饿处理后再重新喂食，已被提议作为一种产生同步化星形胶质细胞的方法。在此，有人提出该方法既不能使细胞同步化，也不符合细胞同步化的严格标准。所提议的非选择性全培养同步化方案，从理论上讲无法使细胞同步化。所提议的血清饥饿/重新喂食方案产生的细胞并不反映细胞周期中任何特定细胞的特性。已发表方案产生的细胞会有广泛的细胞大小分布，因此，饥饿/重新喂食产生的细胞不会模拟分裂周期中任何特定年龄的细胞。所以，所提议的方案不会产生同步化培养物。
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