人类促黑素皮质素-2受体在不同真核细胞中的表达。
Expression of the human melanocortin-2 receptor in different eukaryotic cells.
作者信息
Rached Mohamed, El Mourabit Haquima, Buronfosse Anna, Blondet Antonine, Naville Danielle, Begeot Martine, Penhoat Armelle
机构信息
INSERM, U418/INRA UMR 1245, IFR Laënnec, Lyon F-69005, France.
出版信息
Peptides. 2005 Oct;26(10):1842-7. doi: 10.1016/j.peptides.2004.11.037.
The human melanocortin-2 receptor (hMC2R) is mainly present in the adrenal cortex and has been difficult to express in heterologous cells. The hMC2R fused to the EGFP at its C-terminus has been stably transfected in the murine M3 melanoma and HEK293 cells. In the M3 cells, the hMC2R-EGFP was well-addressed to the cell membrane and functional whereas in the HEK293 cells, the hMC2R-EGFP was retained intracellularly. These results suggest that some specific factors, missing in cells, which do not express any melanocortin receptor, are involved in the correct addressing of the hMC2R to the cell membrane.
人类促黑素皮质素-2受体（hMC2R）主要存在于肾上腺皮质，且难以在异源细胞中表达。C末端融合增强绿色荧光蛋白（EGFP）的hMC2R已在小鼠M3黑色素瘤细胞和人胚肾293（HEK293）细胞中稳定转染。在M3细胞中，hMC2R-EGFP能很好地定位于细胞膜且具有功能，而在HEK293细胞中，hMC2R-EGFP则保留在细胞内。这些结果表明，在不表达任何促黑素皮质素受体的细胞中缺失的某些特定因子，参与了hMC2R向细胞膜的正确定位。
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