5'-聚腺苷酸序列作为真核无细胞系统中翻译的有效引导序列。
5'-poly(A) sequence as an effective leader for translation in eukaryotic cell-free systems.
作者信息
Gudkov Anatoly T, Ozerova Maria V, Shiryaev Vyacheslav M, Spirin Alexander S
机构信息
Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia.
出版信息
Biotechnol Bioeng. 2005 Aug 20;91(4):468-73. doi: 10.1002/bit.20525.
Poly(A) sequence of 25 adenylic residues placed immediately before the start codons of the green fluorescent protein (GFP) and firefly luciferase (Luc) mRNAs is shown to provide a high rate of translation of the heterologous messages in eukaryotic cell-free translation systems. Also the poly(A) leader is found to provide the abolition of the inhibition of translation at excess mRNA concentrations. The possibility of the practical use of the constructs with the poly(A) leader for preparative protein production is demonstrated in the wheat germ continuous-exchange cell-free (CECF) translation system.
在绿色荧光蛋白（GFP）和萤火虫荧光素酶（Luc）mRNA的起始密码子之前紧邻放置的由25个腺苷酸残基组成的聚腺苷酸（Poly(A)）序列，已被证明能在真核无细胞翻译系统中高效翻译异源信息。此外，还发现聚腺苷酸前导序列能够消除在mRNA浓度过高时对翻译的抑制作用。在小麦胚连续交换无细胞（CECF）翻译系统中，证明了带有聚腺苷酸前导序列的构建体用于制备性蛋白质生产的实际应用可能性。
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