Chung Sang-Min, Frankman Ellen L, Tzfira Tzvi
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, 11794, USA.
Trends Plant Sci. 2005 Aug;10(8):357-61. doi: 10.1016/j.tplants.2005.06.001.
Today, cloning vectors that have been specifically designed to facilitate the fusion, overexpression or down-regulation of a variety of genes in plant cells are available from various sources. In most cases, their basic design allows the cloning of a single target gene, typically under a specific promoter, in parallel with the expression of selection and/or marker genes from the same vector. However, new and versatile systems now exist that expand the user's choice to a large number of promoters and terminators, and various autofluorescent tags confer the ability to express multiple genes from a single transformation vector.
如今,专门设计用于促进多种基因在植物细胞中融合、过表达或下调的克隆载体可从多种来源获得。在大多数情况下,其基本设计允许在特定启动子的控制下克隆单个靶基因,同时从同一载体表达选择和/或标记基因。然而,现在已有新型通用系统,可将用户的选择扩展到大量启动子和终止子,并且各种自发荧光标签赋予了从单个转化载体表达多个基因的能力。