通过聚合酶链反应检测B细胞慢性淋巴细胞白血病患者血清中的肿瘤衍生抗原受体DNA。
Detection of tumor-derived antigen receptor DNA by polymerase chain reaction in the serum of patients with B-cell chronic lymphocytic leukemia.
作者信息
Catherwood Mark A, Wall J Paul, Morris T C M Curly, Alexander H Denis
出版信息
Haematologica. 2005 Jul;90(7):992-4.
PMID:15996944
Abstract
Tumor-derived DNA is detectable in the serum of patients. In this study, tumor derived and mononuculear cell DNA from 50 patients with B-cell chronic lymphocytic leukemia (B-CLL) was amplified by polymerase chain reaction (PCR) and analyzed using polyacrylamide electrophoressis and Genescan analysis. DNA was demonstrated in 86% of serum samples. Genescan analysis has significantly enhanced the sensitivity and specificity of detection of tumor-derived DNA.
摘要
肿瘤来源的DNA在患者血清中可检测到。在本研究中，通过聚合酶链反应（PCR）扩增了50例B细胞慢性淋巴细胞白血病（B-CLL）患者的肿瘤来源DNA和单核细胞DNA，并使用聚丙烯酰胺电泳和基因扫描分析进行分析。86%的血清样本中检测到了DNA。基因扫描分析显著提高了肿瘤来源DNA检测的灵敏度和特异性。
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