基于转糖基化反应通过毛细管电泳-飞行时间质谱法解析糖蛋白中的N-连接寡糖。

Resolution of N-linked oligosaccharides in glycoproteins based upon transglycosylation reaction by CE-TOF-MS.

作者信息

Min Jun Zhe, Toyo'oka Toshimasa, Kato Masaru, Fukushima Takeshi

机构信息

Department of Analytical Chemistry, School of Pharmaceutical Sciences, and COE Program in the 21st Century, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka, 422-8526, Japan.

出版信息

Chem Commun (Camb). 2005 Jul 21(27):3484-6. doi: 10.1039/b505744b. Epub 2005 Jun 9.

DOI:10.1039/b505744b

PMID:15997305

Abstract

The resolution of asparagine-type oligosaccharides in glycoproteins was carried out by combination of the transglycosylation reaction and CE-TOF-MS. The oligosaccharides enzymatically transferred to a fluorescent acceptor (NDA-Asn-GlcNAc) with Endo-M. The resulting fluorescent-oligosaccharides were separated by CE and detected by TOF-MS. Disialo-Asn was successfully identified by the proposed procedure. Application to oligosaccharides in ovalbumin was also described in this communication.

摘要

通过转糖基化反应与CE-TOF-MS联用对糖蛋白中的天冬酰胺型寡糖进行拆分。寡糖经内切甘露糖苷酶M酶促转移至荧光受体（NDA-Asn-GlcNAc）上。所得荧光寡糖通过CE分离并由TOF-MS检测。通过所提出的方法成功鉴定出二唾液酸-天冬酰胺。本论文还描述了该方法在卵清蛋白寡糖分析中的应用。