Scalia Frank, Feldheim David A
Department of Anatomy and Cell Biology, State University of New York Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, NY 11203, USA.
Brain Res Dev Brain Res. 2005 Aug 8;158(1-2):102-6. doi: 10.1016/j.devbrainres.2005.05.002.
Eph/ephrin expression was studied in Rana utricularia larvae and adults with in situ receptor and ligand affinity probes. From stages TK-II (early limb bud) to VI (early foot paddle larva), tectal EphB expression is highest in a band extending transversely across the posterior optic tectum and grades off anteriorly and posteriorly. The ephrin-A expression gradient is parallel to the EphB gradient rather than being orthogonal to it. However, its high point occupies the posterior pole, and it runs from high-posteriorly to low-anteriorly. Tectal EphA expression is high anteriorly and low posteriorly, while ephrin-Bs are expressed only in a thin line at the dorsal midline. At later stages and in adults, tectal EphB expression becomes uniform.
利用原位受体和配体亲和探针研究了牛蛙蝌蚪和成年个体中Eph/ephrin的表达情况。从TK-II期（早期肢芽）到VI期（早期蹼足幼虫），视顶盖EphB表达在一条横向穿过视顶盖后部的带中最高，并在前部和后部逐渐减弱。ephrin-A表达梯度与EphB梯度平行，而非与其正交。然而，其高点位于后极，从后高向前低分布。视顶盖EphA表达前高后低，而ephrin-Bs仅在背中线的一条细线中表达。在后期和成年个体中，视顶盖EphB表达变得均匀。
