[多型物种螯虾蛄（Idotea chelipes）和巴氏海蛄虾（Idotea balthica basteri）（甲壳纲等足目）血蓝蛋白的电泳研究]

[Electrophoresis study of hemocyanin of the polytypic species Idotea chelipes and Idotea balthica basteri (Crustacea Isopoda)].

作者信息

Charfi-Cheikhrouha F, Belhadj O

机构信息

Laboratoire de Biologie et de Biochimie, Faculté des Sciences de Tunis, Campus Universitaire, Tunisie.

出版信息

C R Acad Sci III. 1992;314(5):193-7.

PMID:1600462

Abstract

Hemocyanin has been used as a protein marker for Crustacea speciation. The specificity of the hemocyanin fractions of two Idotea species has been confirmed by mono and bidirection electrophoresis. Four protein fractions have been found in the two Idotea species. However, the molecular weight of each Idotea chelipes fraction is slightly higher. The two fractions of high molecular weight are strongly associated. We assumed a molecular weight of 240,000 for I. chelipes and 230,000 for I. balthica basteri. The two other protein fractions are well separated and have a weight of 130,000 and 77,000 in I. chelipes; 120,000 and 75,000 for I. b. basteri. In contrast, the subspeciation of I. chelipes has not been clearly demonstrated by electrophoresis of the hemocyanin.

摘要

血蓝蛋白已被用作甲壳纲物种形成的蛋白质标记物。通过单向和双向电泳已证实了两种艾氏蜾蠃蜚物种血蓝蛋白组分的特异性。在这两种艾氏蜾蠃蜚物种中发现了四种蛋白质组分。然而，每种艾氏蜾蠃蜚螯肢组分的分子量略高。两种高分子量组分紧密相关。我们假定艾氏蜾蠃蜚的分子量为240,000，巴氏艾氏蜾蠃蜚的分子量为230,000。另外两种蛋白质组分在艾氏蜾蠃蜚中分离良好，分子量分别为130,000和77,000；在巴氏艾氏蜾蠃蜚中为120,000和75,000。相比之下，通过血蓝蛋白电泳尚未明确证明艾氏蜾蠃蜚的亚种形成。