Zhu Z, Gonthier R, Neirinck L
Pharmascience, 6111 Royalmount Ave. Suite 100, Montreal, Que., Canada.
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Aug 5;822(1-2):238-43. doi: 10.1016/j.jchromb.2005.06.021.
This paper describes a simple, fast and sensitive liquid chromatography-mass spectrometry method for quantification of an anti-thrombocythemic agent, anagrelide in human plasma. The samples were subjected to a liquid-liquid extraction after addition of a buffer and an internal standard. Chromatography was performed on an Inertsil ODS2 column and the extract was injected onto a HPLC system coupled with mass spectrometric detection. Linear responses for standards were observed from 50 to 7500 pg/ml. The accuracy of intra-assay and inter-assay were in the ranges 4.3-4.4% and 4.8-5.6%, respectively. The method is simple and reproducible with a run time of less than 2 min.
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