Roitto M, Rautio P, Julkunen-Tiitto R, Kukkola E, Huttunen S
MTT Agrifood Research Finland, Environmental Research, Karilantie 2A, FIN-50600 Mikkeli, Finland.
Environ Pollut. 2005 Oct;137(3):603-9. doi: 10.1016/j.envpol.2005.01.046.
Studies were done on the effects of elevated soil concentrations of copper (Cu) and (Ni) on foliar carbohydrates and phenolics in Scots pine (Pinus sylvestris L.). Four year-old seedlings were planted in pots filled with metal-treated mineral forest soil in early June. The experimental design included all combinations of four levels of Cu (0, 25, 40 and 50 mg kg(-1) soil dw) and Ni (0, 5, 15 and 25 mg kg(-1) soil dw). Current year needles were sampled for soluble sugar, starch and phenolics at the end of September. Ni increased sucrose concentration in the needles, indicating disturbances in carbohydrate metabolism. Trees exposed to Ni had higher concentrations of condensed tannins compared with controls. In contrast, concentrations of several other phenolic compounds decreased when seedlings were exposed to high levels of Cu or to a combination of Ni and Cu. The results suggest that concentrations of phenolics in Scots pine needles vary in their responses to Ni and Cu in the forest soil.
开展了关于土壤中铜（Cu）和镍（Ni）浓度升高对苏格兰松（Pinus sylvestris L.）叶片碳水化合物和酚类物质影响的研究。6月初，将4年生幼苗种植在装有经金属处理的矿质森林土壤的花盆中。实验设计包括铜的四个水平（0、25、40和50毫克/千克土壤干重）和镍的四个水平（0、5、15和25毫克/千克土壤干重）的所有组合。9月底采集当年针叶样本，用于分析可溶性糖、淀粉和酚类物质。镍增加了针叶中的蔗糖浓度，表明碳水化合物代谢受到干扰。与对照相比，暴露于镍的树木中缩合单宁的浓度更高。相反，当幼苗暴露于高浓度铜或镍与铜的组合时，其他几种酚类化合物的浓度降低。结果表明，苏格兰松针叶中酚类物质的浓度对森林土壤中的镍和铜的反应各不相同。
