Vani G, Devi C S Shyamala
Department of Biochemistry, University of Madras, Guindy Campus, Chennai, India.
Mol Cell Biochem. 2005 Apr;272(1-2):151-5. doi: 10.1007/s11010-005-6983-3.
The effects of exogenous histone H1 on estrogen receptor status of human breast cancer MCF 7 cells were investigated in presence and absence of estrogen. Exogenous histone H1 was significantly cytotoxic in a dose- and time-dependent manner. Cell cycle analysis revealed a significant increase in the percentage of cell accumulation in G0/G1 phase. In histone H1-treated cells, a significant decrease in the estrogen receptor content and an increase in the dissociation constant (KD) of ER was observed compared to control.
在有雌激素和无雌激素的情况下，研究了外源性组蛋白H1对人乳腺癌MCF 7细胞雌激素受体状态的影响。外源性组蛋白H1具有显著的细胞毒性，呈剂量和时间依赖性。细胞周期分析显示，G0/G1期细胞积累百分比显著增加。与对照组相比，在组蛋白H1处理的细胞中，观察到雌激素受体含量显著降低，雌激素受体解离常数（KD）增加。
