Mohanan Valiya Veettil, Chathu Finla, Paulose Cheramadathikudyil S
Molecular Neurobiology and Cell Biology Unit, Centre for Neuroscience, Department of Biotechnology, Cochin University of Science and Technology, Cochin, India.
Mol Cell Biochem. 2005 Apr;272(1-2):165-70. doi: 10.1007/s11010-005-7030-0.
The purpose of this study was to investigate the role of central 5-HT2C receptor binding in rat model of pancreatic regeneration using 60-70% pancreatectomy. The 5-HT and 5-HT2C receptor kinetics were studied in cerebral cortex and brain stem of sham operated, 72 h pancreatectomised and 7 days pancreatectomised rats. Scatchard analysis with [3H] mesulergine in cerebral cortex showed a significant decrease (p < 0.05) in maximal binding (Bmax) without any change in Kd in 72 h pancreatectomised rats compared with sham. The decreased Bmax reversed to sham level by 7 days after pancreatectomy. In brain stem, Scatchard analysis showed a significant decrease (p < 0.01) in Bmax with a significant increase (p < 0.01) in Kd. Competition analysis in brain stem showed a shift in affinity towards a low affinity. These parameters were reversed to sham level by 7 days after pancreatectomy. Thus the results suggest that 5-HT through the 5-HT2C receptor in the brain has a functional regulatory role in the pancreatic regeneration.
本研究的目的是利用60 - 70%胰腺切除术的大鼠模型，研究中枢5 - HT2C受体结合在胰腺再生中的作用。对假手术、胰腺切除72小时和胰腺切除7天的大鼠的大脑皮层和脑干中的5 - HT和5 - HT2C受体动力学进行了研究。用[3H]美舒麦角在大脑皮层进行的Scatchard分析显示，与假手术组相比，胰腺切除72小时的大鼠最大结合量（Bmax）显著降低（p < 0.05），解离常数（Kd）无变化。胰腺切除术后7天，降低的Bmax恢复到假手术水平。在脑干中，Scatchard分析显示Bmax显著降低（p < 0.01），Kd显著升高（p < 0.01）。脑干中的竞争分析显示亲和力向低亲和力方向转变。这些参数在胰腺切除术后7天恢复到假手术水平。因此，结果表明大脑中的5 - HT通过5 - HT2C受体在胰腺再生中具有功能调节作用。
