Hughes P L, Lawrence J E, Scott S W
Department of Entomology, Soils, and Plant Sciences, Clemson University, Clemson, South Carolina 29634, USA.
Arch Virol. 2005 Nov;150(11):2339-45. doi: 10.1007/s00705-005-0594-x. Epub 2005 Jul 14.
The complete nucleotide sequence of the genome of the potexvirus, hydrangea ringspot virus, has been determined. The sequence is 6,185 nt in length, excluding the poly (A) tail, and contains six ORFs coding for proteins of 156, 26, 12, 8, 24, and 16 kDa, respectively. ORF 6 is contained within ORF 5 and in this respect the virus is similar to the potexviruses CsCMV, NMV, and SMYEV. Phylogenetic analysis of the putative products of the ORFs and signature motifs contained within these products shows the virus to be most closely related to CsCMV. A similar analysis of data for the coat proteins of potexviruses did not support the previously reported serological relationships between HdRSV and other potexviruses. This is the first complete sequence published for the genome of a potexvirus infecting a dicotyledonous, temperate, deciduous woody species.
已确定了马铃薯X病毒属的绣球花环斑病毒基因组的完整核苷酸序列。该序列长度为6185个核苷酸(不包括聚腺苷酸尾),包含六个开放阅读框,分别编码156、26、12、8、24和16千道尔顿的蛋白质。开放阅读框6包含在开放阅读框5内,在这方面该病毒与马铃薯X病毒CsCMV、NMV和SMYEV相似。对这些开放阅读框的推定产物及其所含特征基序进行系统发育分析表明,该病毒与CsCMV关系最为密切。对马铃薯X病毒外壳蛋白数据的类似分析并不支持先前报道的HdRSV与其他马铃薯X病毒之间的血清学关系。这是首次发表的感染双子叶、温带、落叶木本植物的马铃薯X病毒基因组的完整序列。